Journal: Materials Today Bio

Article Title: Neuroactive network tissue based on dual-factor neuroregenerative bioactive coating scaffolds and neural stem cells for spinal cord injury repair

doi: 10.1016/j.mtbio.2025.102172

Figure Lengend Snippet: Preparation and bioactivity evaluation of immobilized recombinant growth factors and the construction of the neuroregenerative coating fiber network scaffold. (A) SDS-PAGE gel showed the preparation process of immobilized recombinant growth factors D-IGF1 and D-NGF including: marker (M), pre-induction (1), post-induction (2), supernatant after centrifugation (3), pellet after centrifugation (4), refolded protein solution (5), flow-through liquid (6), wash liquid (7), elution liquid (8). (B) The effect of different densities of D-IGF1 and D-NGF on NSC proliferation. (C–D) The effect of different densities of D-IGF1 and D-NGF on neural stem cell differentiation. Scale bars, 100 μm (E) SEM images of the surface morphology of fiber scaffolds prepared under different process parameters. Scale bars, 10 μm (F–G) Biocompatibility testing of the scaffold. (H) Contact angle analysis. (I) FT-IR analysis. (J) XPS analysis. (Data are shown as the mean ± SD, n = 3, ∗p < 0.05,∗∗p < 0.01).

Article Snippet: The resulting cells were resuspended in NSC proliferation medium containing DMEM/F12 (Gibco, A4192001, USA), 1 % penicillin-streptomycin (PS, Sigma-Aldrich, USA), 1 % glutamine (Gibco, USA), 20 ng mL −1 basic fibroblast growth factor (bFGF, Solarbio, China), and 20 ng mL −1 epidermal growth factor (EGF, Solarbio, China).

Techniques: Recombinant, SDS Page, Marker, Centrifugation, Cell Differentiation